CONTENIDO: I - INTRODUCTION
1 - What is a Bioprocess Engineer?
Introductory Remarks - Biotechnology and Bioprocess Engineering - Biologists and Engineers Differ in Their Approach to Research - The Story of Penicillin: How Biologists and Engineers Work Together - Bioprocesses: Regulatory Constraints - Suggestions for Further Reading - Problems -
II - THE BASICS OF BIOLOGY: AN ENGINEER'S PERSPECTIVE
2 - An Overview of Biological Basics -
Are All Cells the Same? Cell Construction - Cell Nutrients - Summary - Suggestions for Further Reading - Problems -
3 - Enzymes
Introduction - How Enzymes Work - Enzyme Kinetics - Immobilized Enzyme Systems - Large-scale Production of Enzymes - Medical and Industrial Utilization of Enzymes - Summary - Suggestions for Further Reading - Problems -
4 - How Cells Work -
Introduction - The Central Dogma - DNA Replication: Preserving and Propagating the Cellular Message - Transcription: Sending the Message - Translation: Message to Product - Metabolic Regulation - How the Cell Senses Its Extracellular Environment - Summary - Appendix: Examples of Regulation of Complex Pathways - Suggestions for Further Reading - Problems -
5 - Major Metabolic Pathways
Introduction - Bioenergetics - Glucose Metabolism: Glycolysis and the TCA Cycle - Respiration - Control Sites in Aerobic Glucose Metabolism - Metabolism of Nitrogenous Compounds - Nitrogen Fixation - Metabolism of Hydrocarbons - Overview of Biosynthesis - Overview of Anaerobic Metabolism - Overview of Autotrophic Metabolism - Summary - Suggestions for Further Reading - Problems -
6 - How Cells Grow
Introduction - Batch Growth - Quantifying Growth Kinetics - How Cells Grow in Continuous Culture - Summary - Suggestions for Further Reading - Problems 7 - Stoichiometry of Microbial Growth and Product Formation
Introduction - Some Other Definitions - Stoichiometric Calculations - Theoretical Predictions of Yield Coefficients - Summary - Suggestions for Further Reading - Problems
8 - How Cellular Information is Altered
Introduction - Evolving Desirable Biochemical Activities through Mutation and Selection - Natural Mechanisms for Gene Transfer and Rearrangement - Genetically Engineering Cells - Genomics - Summary - Suggestions for Further Reading - Problems
III - ENGINEERING PRINCIPLES FOR BIOPROCESSES
9 - Operating Considerations for Bioreactors for Suspension and Immobilized Cultures
Introduction - Choosing the Cultivation Method - Modifying Batch and Continuous Reactors - Immobolized Cell Systems - Solid-state Fermentations - Summary - Suggestions for Further Reading - Problems
10 - Selection, Scale-Up, Operation, and Control of Bioreactors
Introduction - Scale-up and Its Difficulties - Bioreactor Instrumentation and Control - Sterilization of Process Fluids - Summary - Suggestions for Further Reading - Problems-
11 - Recovery and Purification of Products
Strategies to Recover and Purify Products - Separation of Insoluble Products - Cell Disruption - Separation of Soluble Products - Finishing Steps for Purification - Integration of Reaction and Separation - Summary - Suggestions for Further Reading - Problems
IV - APPLICATIONS TO NONCONVENTIONAL BIOLOGICAL SYSTEMS
12 - Bioprocess Considerations in Using Animal Cell Cultures
Structure and Biochemistry of Animal Cells - Methods Used for the Cultivation of Animal Cells - Bioreactor Considerations for Animal Cell Culture - Products of Animal Cell Cultures - Summary - Suggestions for Further Reading - Problems - 13 - Bioprocess Considerations in Using Plant Cell Cultures -
Why Plant Cell Cultures? Plant Cells in Culture Compared to Microbes - Bioreactor Considerations - Economics of Plant Cell Tissue Cultures - Summary - Suggestions for Further Reading - Problems -
14 - Utilizing Genetically Engineered Organisms -
Introduction - How the Product Influences Process Decisions - Guidelines for Choosing Host-Vector Systems - Process Constraints: Genetic Instability - Considerations in Plasmid Design to Avoid Process Problems - Predicting HostÐVector Interactions and Genetic Instability - Regulatory Constraints on Genetic Processes - Metabolic Engineering - Protein Engineering - Summary - Suggestions for Further Reading - Problems -
15 - Medical Applications of Bioprocess Engineering
Introduction - Tissue Engineering - Gene Therapy Using Viral Vectors - Bioreactors - Summary - Suggestions for Further Reading - Problems
16 - Mixed Cultures
Introduction - Major Classes of Interactions in Mixed Cultures - Simple Models Describing Mixed-culture Interactions - Mixed Cultures in Nature - Industrial Utilization of Mixed Cultures - Biological Waste Treatment: An Example of the Industrial Utilization of Mixed Cultures - Summary - Suggestions for Further Reading - Problems -
17 - Epilogue
Appendix: Traditional Industrial Bioprocesses
Anaerobic Bioprocesses - Aerobic Processes
Suggestions for Further Reading
Index
